Interaction of nitrobenzoxadiazole derivatives of piperazine and aniline with bovine serum albumine in silico and in vitro

Albumin is a globular protein of plasma mammalian blood participating in transport hydrophobic metabolites and drugs. Thus, studies devoted to its binding are valuable as part evaluation new potential drugs or fluorescent probes for vivo usage. Here we describe results concerning synthesis bovine serum albumin assay both silico (docking) vitro (spectrophotometric spectrofluorimetric titrations) four 7-nitrobenzoxadiazol-4-yl (NBD) derivatives aniline piperazine. Experimental dissociation constant NBD-ethynylaniline 4 was calculated be about 10 µmol/L.